Salk Institute for Biological Studies

The part-time Intern position is intended to learn and assist with routine lab support. Under general supervision, activities include: collects and prepares mouse DNA samples for analysis; prepares solutions and media under sterile conditions;  performs various molecular biology techniques; and assists with general laboratory maintenance, including equipment maintenance and ordering/stocking of supplies.  ESSENTIAL FUNCTIONS (70%) Collection/Preparation of mice DNA samples for analysis Collects and prepares mouse DNA tissue samples for analysis. Handles and restrains mice. Learns principles of and assists with management of mice colony. (30%) Research Support Performs various techniques in molecular biology such as: DNA extraction and analysis and PCR reactions. Performs inventory of chemicals and supplies; orders laboratory supplies and stocks laboratory areas as required. Operates sterilization equipment ensuring appropriate...

The Research Assistant I position is responsible for performing various genetic and molecular biology techniques, fruit fly handling, stock maintenance, behavior assay under different treatments through the aging process in support of the research goals of the Panda lab. Other duties include assisting with general laboratory maintenance. ESSENTIAL FUNCTIONS (40%) fly stock maintaining and general fly work Provide fresh food to lab stocks routinely and maintain the stocks at healthy condition Carry out fly mating experiment with basic genetic techniques to obtain fly with target genotype (40%) conducting experiments with flies under different feeding regimens Provide and remove food from experimental flies according to different schedules Conduct different types of fly behavior assays and analyze the data under different treatments Dissect tissues and perform molecular and immunohistological analysis (10%) Molecular Biology...

The Law lab focused on chromatin and gene regulation and the CRoPS project aims to leverage these processes to develop crop plants that sequester more carbon via extensive root systems containing recalcitrant carbon polymers to fight climate change. A major component of this research is to conduct genetic and genomic analysis for understanding traits relevant for plant improvements. The Research Asst I will work closely with a Research Asst III or Post Doctoral fellow, and other members of the CRoPS project and the Law laboratory to preform various molecular biology and microscopy techniques in support of research projects aimed and understanding gene regulation at the genetic and epigenetic levels. The RAI will work in a team responsible for (1) phenotyping Arabidopsis thaliana lines of interest (2) screening, genotyping, and maintaining plant lines (3) designing constructs to assess gene function and expression and (4) staining and preparing plant samples...

POSITION SUMMARY The Chory lab is looking for a Research Assistant I, under supervision, to characterize the effect of a microRNA on Arabidopsis root development.  Responsibilities include: molecular biology experiments, microscopy, plant maintenance and manipulation, optimizing protocol for plant growth, data analysis, and troubleshooting.  ESSENTIAL FUNCTIONS (100%) Research and Lab Support Conducts molecular cloning and generates transgenic Arabidopsis plants. Root imaging. Phenotypes plants and evaluates plant robustness. Performs RNA extractions and gene expression analysis. Performs plant tissue dissection. Assists with maintaining lab stocks. EXPERIENCE Required: No professional experience required with relevant degree Preferred: Previous experience in handling Arabidopsis; Previous research experience in PCR, DNA manipulations, electrophoresis, and bacteria transformation EDUCATION Required:...

Under direction, the intern will perform tasks and support of academic research projects. Responsibilities may include: maintaining C. elegans stocks, preparing reagents, genotyping, performing behavior experiments, data entry and analysis, and giving presentations. ESSENTIAL FUNCTIONS (100%) Lab Support Maintaining nematode stocks Preparing reagents Genotyping Performing behavior experiments Data entry Data analysis Presentations EXPERIENCE   Required:   No professional experience required   Preferred: Basic use of computer programs (Word, Excel) EDUCATION   Required:   High School Diploma or equivalent work experience Preferred:   Undergraduate coursework in life sciences or equivalent work experience SKILLS AND ABILITIES Required:   Punctual, meticulous and rigorous in completing an assigned task.  Preferred :   Theoretical knowledge of biological sciences....

POSITION SUMMARY We are seeking a highly motivated Research Assistant (early or established stage) with a strong interest in one or more of the following: 1) development of oncolytic viruses using an adenovirus-based platform, 2) research in DNA damage response pathway upon viral infection, and 3) developing human organoids derived from induced-human pluripotent stem cell (iPSC). Our group develops and applies state-of-the-art viral genome engineering and editing tools, and deep microscopy studies to address fundamental unanswered biological questions with therapeutic relevance at the forefront of virology and molecular biology research. Our laboratory is equipped to conduct virology experiments at BioSafety Level (BSL) 1 and 2. The Research Assistant will assist a senior lab member in the development of novel tools designed to facilitate novel therapeutic applications. The Research Assistant will perform molecular and cellular biology and virology...

  Application Instructions: Employment History   Include your last ten (10) years of employment history, or length of your employment history if less, including all position (even those that are not relevant to this position) and periods of unemployment. Incomplete information could disqualify you from further consideration. POSITION DESCRIPTION This position is responsible for the basic daily husbandry operations in all facilities and with all species in accordance with standard operating procedures and training to ensure compliance with the Guide for the Care and Use of Laboratory Animals, the Animal Welfare Act and Regulations as well as all applicable federal, state, local and institutional laws, regulations, policies  ESSENTIAL FUNCTIONS Husbandry (75%) AHT II, LEVEL A: PROVIDES ADVANCED CARE AND HUSBANDRY FOR ALL RODENT SPECIES Performs daily health and husbandry observations, change cages, flood and rack line...

The candidate will lead a team of analytical researchers to design, build and deploy an advanced MS-based platform for the high throughput analysis of plant and animal tissues, including pipelines for the extraction of isotope enrichment data from tracer studies using 13C, 2H, or 15N isotopes. The position will develop and deploy mass spectrometry (MS) based, quantitative measurements of plant tissue polymers including but not limited to suberin, lignin and (hemi)cellulose and associated biomarkers for each at high throughput across a variety of row and cover crops to support HPI's CRoPS (CO2 Removal on a Planetary Scale) program. In this position, the candidate will have the opportunity to develop and improve MS data acquisition, throughput and quantitative interpretation using pyrolysis gas chromatography-mass spectrometry (pyGC-MS) as well as other MS-based modalities to assess biopolymer distributions in plant and animal tissues. CRoPS is a...

  Under general direction, incumbent is responsible for fulfilling administrative support needs of the Pre & Postdoctoral Office and Chief Science Office, which include a variety of technical and confidential duties related to academic trainees. The incumbent will be assigned a variety of tasks in support of these activities, but be expected to think through them and their reflection on the department when performing them. The position requires a skill set that includes strong organizational and time management skills, excellent oral and interpersonal skills, and the ability to assess and manage shifting priorities and adjust workload accordingly in order to meet all expected deadlines. ESSENTIAL FUNCTIONS Academic Administration (50%) Monitors time in training for postdocs, scheduling reminder notices for training periods, and coordinating changes to staff positions or terminations with HR as necessary.  Semi-annually reviews training limits...

  The Research Assistant II will be responsible for, under the supervision of a postdoctoral associate, research support and some lab management duties in support of the Principal Investigator's (PI) studies. Research duties will involve performing techniques in molecular biology and biochemistry such as production and purification of nucleic acids, proteins, and nucleoprotein complexes. Scientific research duties will also include performing biochemical assays in support of research projects as well as assisting the postdoctoral associates with experimental design and direction of future experiments. This position will also involve assisting in hiring new staff as well as leading and assisting with training of junior lab staff (junior technicians, students and volunteers) as necessary. The position provides opportunities for some independence in defining the directions of the project, under general guidance by a postdoctoral associate and principal...

The Michael Group in the Plant Molecular and Cellular Biology Laboratory at the Salk focuses on sequencing, assembling and analyzing plant genomes. A broad objective is to build genome resources for plants with unique morphological or lifestyle characteristics (carnivorous, parasitic), minimal plants (duckweed), and crop wild relatives (CWR), to better understand plant genome organization and trait architecture. Currently we are working on several different strategies to generate chromosome-level haplotype-resolved polyploid genomes. In addition, we are developing new techniques to annotate (coding and non-coding) non-model plant genomes using single-cell sequencing, direct DNA modification detection, and computational approaches. We are developing reference-free pan-genome methods for integration into genome wide selection studies (GWAS) and genomic selection paradigms to link population level phenotypes to gene networks underlying environmental response and...

The Michael Group in the Plant Biology Laboratory at the Salk Institute focuses on sequencing and analyzing plant genomes. The Bioinformatics Analyst I (BAI) position will work closely with the PI and other members of the lab to analyze plant genome data generated in the lab. The BAI will be responsible for executing and maintaining current analysis pipelines, building new analysis pipelines, and integrating new genomics tools when appropriate. The BAI will also work closely with members of the lab and larger Plant Biology group to interpret genomics data as well as assist in non-pipeline bioinformatics applications. There will be a specific focus on assembling plant genomes from Pacific Bioscience (PacBio) and Oxford Nanopore Technologies (ONT) long read sequencing technologies. The BAI will stay current on best practices and update the genome assembly pipelines according to advances in the sequencing technologies. The BAI will develop gene prediction...

  Job location  is at  10240 Science Center Dr,  San Diego, CA 9212 The role of the incumbent is to advise, recommend and further avenues to advance diversity at Salk, working closely with other members of the Office of Equity & Inclusion (OEI), partners (e.g., Human Resources (HR), Education Outreach (EO), External Relations (ER), Grants Development, the Postdoctoral Office (PDO), the Chief Science Officer (CSO), the Advisory Council on Diversity (ACOD)), and Salk stakeholders (e.g., Salk faculty and employees). With general direction from the VP, Equity & Inclusion (the Institute's chief diversity officer), the incumbent will plan, support, and promote objectives to support increased diversity through outreach and pathway development, recruitments, funding and learning to move forward the Institute's DEI strategic plan. ESSENTIAL FUNCTIONS Research, Strategy & Execution/Implementation - 50% Through published...

The incumbent will assist with molecular/cellular biological techniques including freezing and thawing cells, RNA/DNA extractions, DNA analysis, cloning, PCR, running gels, transcription reactions, transient transfections, flow cytometry, and CRISPR gene editing. ESSENTIAL FUNCTIONS 100% Research Support Perform and assist with molecular biology experiments Work closely with postdoctoral scholars and graduate students Present experimental results during laboratory meetings EXPERIENCE Required: No professional experience required Preferred: 1-3 years of related experience EDUCATION Required: Bachelor's degree in biological sciences, life sciences or related field Preferred: SKILLS AND ABILITIES Required: Human cell culture in a BSL2+ environment Cloning and construction of plasmid libraries PCR, electrophoresis and other basic molecular biology techniques Transfection and viral transduction...

POSITION SUMMARY The Research Assistant II position will focus on brain slicing and dissection, nuclei production and sorting for multiomics analysis, protein biochemistry and image analysis.  The successful candidate will work approximately 75% of the time on funded projects and the rest on support postdoctoral researchers in the lab. Additionally, the position will be responsible for establishing conditions and running spatial transcriptomic analysis using MERFISH.  The position will require training in brain slicing, nuclei production and sorting, neuronal imaging and analysis, and in specific techniques and methods in order to execute experiments that are broadly designed by the PI or postdoctoral researchers.  Following training, the successful candidate must be able to carry out and analyze experimental results independently with limited instruction for routine work.  The overall goal of the research is to use epigenomics to create an ATLAS of the...

POSITION SUMMARY The Michael Group in the Plant Biology Laboratory (PBIO) at the Salk Institute focuses on sequencing and analyzing plant genomes. As part of the focus on plant genomics, the group maintains capabilities in high molecular weight (HMW) DNA extraction, RNA extraction, sequencing library generation, running of sequencing platforms and methods for generating chromosome-resolved assemblies (HiC, BioNano). In addition, there is a focus on applying current genomics tools to further the understanding of plant genome structure and function. Furthermore, as new genomics tools emerge, the group routinely tests these new methods to assess if they could play a role in improving plant genome sequencing, assembling and annotation. The Michael Group wet lab works very closely with the computational dry lab to reach the goals of high-quality plant genomes. The Research Assistant III will be responsible for the sequencing wet lab in the Michael Group. The...

The Michael Group in the Plant Biology Laboratory (PBIO) at the Salk Institute focuses on sequencing and analyzing plant genomes. As part of the focus on plant genomics, the group maintains capabilities in high molecular weight (HMW) DNA extraction, RNA extraction, sequencing library generation, running of sequencing platforms and methods for generating chromosome-resolved assemblies (HiC, BioNano). In addition, there is a focus on applying current genomics tools to further the understanding of plant genome structure and function. Furthermore, as new genomics tools emerge, the group routinely tests these new methods to assess if they could play a role in improving plant genome sequencing, assembling and annotation. The Michael Group wet lab works very closely with the computational dry lab to reach the goals of high-quality plant genomes. The Research Assistant I (RAI) will assist with the sequencing wet lab in the Michael Group. The RAI will work with other...

This position will be assisting senior lab members and graduate students in support of academic research projects. The work will be a combination of experimental work and data analysis. The experimental aspects will primarily include molecular and cellular biology works such as cell culture, protein, DNA and RNA expression studies, cloning, and microscopy works. The data analysis aspects will include processing of imaging and sequencing data.  Prior experience with wet lab techniques is highly preferred. The intern will be trained to design and conduct molecular and cellular biology, and some virology studies. ESSENTIAL FUNCTIONS Lab Support  Responsible for the preparation of reagents and research chemicals Responsible for stocking and organizing incoming lab supplies Responsible for cleaning glassware and equipment as needed Molecular and Cellular Biology  Isolating DNA, RNA, and Protein from cells Generating plasmids by DNA cloning...

The Michael Group in the Plant Biology Laboratory (PBIO) at the Salk Institute focuses on sequencing and analyzing plant genomes. As part of the focus on plant genomics, the group maintains capabilities in high molecular weight (HMW) DNA extraction, RNA extraction, sequencing library generation, running of sequencing platforms and methods for generating chromosome-resolved assemblies (HiC, BioNano). In addition, there is a focus on applying current genomics tools to further the understanding of plant genome structure and function. Furthermore, as new genomics tools emerge, the group routinely tests these new methods to assess if they could play a role in improving plant genome sequencing, assembling and annotation. The Michael Group wet lab works very closely with the computational dry lab to reach the goals of high-quality plant genomes. The Research Assistant II (RAII) will perform tasks for the sequencing wet lab in the Michael Group. The RAII will work...

POSITION SUMMARY   The Research Assistant I position, with general instruction on routine work, will carry out a variety of basic and specialized molecular biology techniques in support of the lab goals. Duties include: focus on brain slicing and dissection, nuclei production and sorting for multiomics analysis, protein biochemistry and image analysis. The successful candidate will work approximately 75% of the time on funded projects and the rest on support postdoctoral researchers in the lab. Additionally, the position will be responsible for establishing conditions for spatial transcriptomics using MERFISH. The position will require training in brain slicing, nuclei production and sorting, neuronal imaging and analysis, and in specific techniques and methods in order to execute experiments that are broadly designed by the PI or postdoctoral researchers. Following training, the successful candidate must be able to carry out and analyze experimental...