Research Assistant I, Gene Expression Laboratory, Dr. Evans

We are seeking a motivated candidate to join our cancer research group in the Evans laboratory. The position will support efforts to identify and target epigenetic programs that contribute to therapeutic resistance and immunosuppression in pancreatic cancer. The majority of the time will be dedicated to the conduct and analysis of experiments and will require the candidate to become adept at a broad range of cellular and molecular biology techniques, such as RNA isolation, qPCR, immunohistochemistry, flow cytometry, and cell culture, that will support analysis of both cutting edge mouse models and primary human patient specimens.

The ideal candidate will be experience in at least some of these techniques, enthusiastic about learning new technologies, and willing to work with animal models of disease. Come join our highly collaborative team as we tackle some of the toughest challenges in pancreatic cancer.

ESSENTIAL FUNCTIONS

(50%) Molecular and Cellular Biology, and Biochemistry Techniques

• Plasmid DNA ( Maxi and mini) and RNA preparation from tissues and cells
• Gel Electrophoresis ( DNA, RNA, Protein ( SDS-page, Western Blotting)
• Molecular cloning
• Analyze RNA using Agilent BioAnalyzer
• PCR/ qPCR in 384 -well format
• cDNA synthesis from RNA
• DNA/RNA sequencing and analysis
• Cutting /slicing mouse tissues ( using microtome /cryostat) for IHC
• Lentiviral vector production 

(20%)  Mouse Colony Handling and Maintenance

• Mouse husbandry/ feeding with special diet/ weaning , mating, breeding
• Weighin/ Euthanization / Mouse survival surgery
• Dissection of adult and embryonic mouse tissues
• Tissue processing for histology
• Tail clipping/ ear punching
• Genotyping
• Behavioral studies
• Tissue processing for flow cytometry

(20%) Tissue Culture

• Maintain Tissue Culture Cells / harvesting/ freezing/drug treatment

(10%) Lab Support

• Assist with preparing standard media and solutions.
• Assist with weekly inventory and ordering of lab supplies.
• Assist with receipt and organization of incoming lab supplies.
• Clean glassware and equipment as needed.
• Assist in disposal of hazardous, biohazard waste and chemical waste

EXPERIENCE

Required:

• No professional experience required with relevant degree.
• Demonstrated experience and application of molecular biology knowledge preferably in biochemistry and cell culture.
• Demonstrated experience with general lab maintenance, including cleaning, stocking supply inventory, and ensuring the maintenance of a sterile environment.

Preferred: No further experience preference.

EDUCATION

Required:

• Bachelor's degree in biological sciences or related field.

Preferred: No further education preference.

SKILLS AND ABILITIES

Required:

• Theoretical and hands on knowledge of tissue culture and molecular biology techniques.
• Demonstrated knowledge and experience in researching information and analyzing data to arrive at valid conclusions.
• Ability to learn and carry out new experimental procedures with accuracy and efficiency. Demonstrated ability to follow verbal and/or written protocols, including follow-up on previously begun procedures.
• Demonstrated ability to record data accurately and enter into appropriate medium.
• Skill in the operation and maintenance of various lab equipment.
• Demonstrated knowledge and experience with both PC and MAC operating systems, standard business office equipment and commonly used software and tools, such as Microsoft Office, electronic mail, and the internet.
• Proficiency with common office software applications as well as software applications used for research, recording, and analysis.
• Highly organized, effective at multitasking, adept at juggling changing priorities
• Proven ability to work both independently and also as part of a collaborative team.
• Excellent communication skills, both oral and written.
• Strong interpersonal skills, including tact, diplomacy, and flexibility.
• Demonstrated ability to interact effectively with diverse groups. Demonstrated skill in establishing and maintaining effective working relationships with personnel at all levels.

Preferred: No further skills preferred.

SPECIAL CONDITIONS OF EMPLOYMENT

• Must be willing to work in an animal-related research environment.
• Must be willing to work an adjusted schedule to support research needs.
• Successful completion of the Institute's background investigation.
• Must be willing to sign a confidentiality agreement.

PHYSICAL REQUIREMENTS/MENTAL ACTIVITIES/ENVIRONMENTAL CONDITIONS

The incumbent in this position will be constantly adjusting focus, seeing, sitting, touching/feeling, and working indoors.