Flow Cytometry Assistant

  • Salk Institute for Biological Studies
  • La Jolla, CA 920371002, USA
  • May 17, 2022
Full time Biology/Biotechnology/Life Sciences

Job Description

ESSENTIAL FUNCTIONS
FCCF Core Facility Training and Support (50%)

  • Provide basic orientation for new users for cytometers and assist with set-up and execution of simple experiments
  • Work closely with researchers from multiple laboratories and scientific backgrounds simultaneously
  • Provide training and assistance to new and existing facility users regarding routine instrument operation, panel design, experiment troubleshooting, and data analysis. Provide assistance and training in the management, processing and analysis of simple and to moderately complex flow cytometry data. Consults with more experienced FCCF staff on more complex troubleshooting and analysis matters.
  • Assist core director and staff with cell sorter startup according to appointment schedule and provide support with cell sorting efforts in the core as needed
    Execute performance verification and tracking procedures for analytical cytometers
  • Perform routine maintenance and cleaning of instruments including sterility testing for sorters (including culturing cells that have undergone sorting)
  • Communicate effectively with other team members and facility users
  • Ensure users are aware of and compliant with appropriate data analysis methods and ethics, escalating concerns for supervisory staff to address as needed
  • Assists facility users in routine multi-parameter data acquisition and analysis using various software including: Diva acquisition software, FlowJo.

FCCF Core Facility Maintenance and Management (40%)

  • Keep database of scientific core facility records such as equipment usage and report maintenance issues of various equipment
  • Perform general maintenance of instrumentation and core resources and ensure assigned areas, and various pieces of equipment are maintained
  • Restock and order lab supplies and reagents
  • Maintaining a clean, orderly work area (dispose of lab waste and chemical waste as appropriate) and prepare buffers and reagents
  • Call for service/liaise with engineers on instrumentation issues
  • Use scheduling software to prepare monthly invoices for facility user recharges and to keep track of resource usage to prepare monthly status reports for Core Director
  • Assist in ensuring core facility is in compliance with EH&S protocols and procedures
  • As opportunities permit and/or as advised by supervisor(s), keep informed of new developments and technical advances in the bioinformatics and genomics fields by reading primary literature and vendor websites

Data Collection and Presentation (10%)

  • Assist in preparing data for publication, grant applications, posters and other presentations using digital facilities
  • With guidance from more experienced staff, present data at department or Institute meetings
  • Prepare images for presentations
  • Assist in core director in the preparation of manuscripts, grants, progress reports and annual protocols
  • Maintain lab notebook, including experimental design, time, methods, materials and results

EXPERIENCE

Required:

  • Typically requires a minimum of 6 months of experience in flow cytometry techniques and principles of operation
  • Experience preparing samples, with basic direction, for flow cytometry and data acquisition/analysis of samples
  • Basic experience with operation of BD analyzers and FACSDiva platforms

Preferred:

  • 1 or more years of experience in flow cytometry techniques and principles of operation
  • Experience with operation of BD Influx cell sorter and BD Aria cell sorter
  • Prior experience working in flow cytometry core facility environment
  • Experience with Flowjo analysis software
  • Experience analyzing FACS data

EDUCATION

Required:

  • Bachelors degree in biological sciences, bioengineering or related discipline, or equivalent work experience

Preferred:

  • Advanced degree in biological sciences, bioengineering or related discipline
  • SKILLS AND ABILITIES

Required:

  • Theoretical knowledge of physics, fluidics, optics, biology, molecular biology, immunochemistry, and information technology at the bachelors level, with ability to apply theoretical knowledge to routine practical analysis.
  • Ability to learn and design routine technical and analytic approaches to cytometry-based experiments.
  • Basic knowledge in flow cytometry data analysis strategies, and ability to learn common flow cytometry data analysis software such as Flow Jo.
  • Skill and ability to perform antibody staining for cell identity, cell sorting, cell cycle profiling and apoptotic assays
  • Ability to utilize multiple computer/instrument software interfaces, cytometric software and basic computer software. Basic experience handling and analyzing large complex datasets.
  • Experience conducting basic research studies.
  • Ability to logically organize and maintain data so it is easily retrievable in electronic and other forms.
  • Strong suite of organizational, management, and analytical skills. Demonstrated ability to analyze scientific, technical and other specialized information. Experience prioritizing tasks and goals in a highly changeable environment. The ability to efficiently assess a broad spectrum of technical and resource requirements with the proven skill to identify, define, and delegate the efforts necessary to address those requirements in a high-performance setting.
  • Excellent demonstrated written and oral communication skills, and experience using graphic display of data to convey scientific information, grant writing, progress reports, and documentation of protocols.
  • Excellent customer service and interpersonal skills. Interpersonal skills include tact, good judgment, diplomacy and flexibility to relate to a diverse group of scientific research staff, faculty, executive management, administrative staff and external contacts.

Preferred:

  • Experience operating a flow cytometer
  • Basic knowledge of Flowjo analysis software
  • Knowledge and use of photonics, fluidics, and lasers.
  • Skill and ability in single cell isolation, membrane potential experiments, stem cell purifying techniques, intracellular protein expression analysis.
  • Demonstrated excellence in instruction of lab techniques and in management of complex technological core facilities.
  • Demonstrated ability to teach/train research methods and techniques to graduate students, postdoctoral researchers, scientists and others as needed /requested and identify when additional training is needed.

SPECIAL CONDITIONS OF EMPLOYMENT

  • Must be willing to work in an animal-related research environment.
  • Must be willing to work in a biohazardous environment (BSL-2).
  • Must be able to work with high voltage equipment.
  • Able to work a flexible schedule based on research needs and deadlines.
  • Satisfactory completion of the Institute's background investigation.
  • Must be willing to sign a confidentiality agreement.

PHYSICAL REQUIREMENTS/MENTAL ACTIVITIES/ENVIRONMENTAL CONDITIONS

This position will constantly be adjusting focus, grasping, handling, hearing, keying, seeing, sitting, talking, analyzing, calculating, communicating, reading, reasoning, writing, and working inside.

Application Details

Apply Here: https://www.salk.edu/about/careers/

 

 Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities

 

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