Molecular Biology/Electrophysiology lab seeks a post-doctoral fellow to carry out exciting research on the development of synchronous activity of vertebrate neurons in culture and in vivo. Responsibilities include conducting experiments involving cell culture, molecular genetics, multi-electrode array (MEA) electrophysiology and computational analyses. The project is recently funded by NSF and is an active collaboration between DSU (PI- Dr. Murali Temburni) and Georgetown University (Co-PI Dr. Rhonda Dzakpasu). The position is available immediately and will remain open until filled. Please apply with a recent CV and list of references to
Dr. Murali Temburni,
Biological Sciences Department
Delaware State University
Summary of the Research Project:
My lab is interested in understanding the role of astrocyte-neuron interaction in the development of neuronal synchrony. Our preliminary data using mixed neuron and astrocyte cultures on multi-electrode arrays (MEAs) showed random spiking activity which synchronizes over time. In comparison, astrocyte-free neuronal cultures only show random activity without synchronization. We are now focusing on astrocytic release of glutamate – mediated by the mGluR1 G-protein-coupled-receptor (GPCR) pathway as a mechanism for the development of neuronal synchronous activity. A model for the mechanism by which the astrocyte mGluR1 pathway mediates neuronal synchronization will be tested using several different dominant negative constructs. A dominant-negative mGluR1 receptor that blocks downstream signaling will be used to understand this signaling pathway’s role in both population synchrony, and in the temporal relationship between calcium oscillations within astrocytes and the development of neuronal synchronous bursts. A dominant-negative SNARE protein Vamp2/Syb2 will be used to block glutamate release from astrocytes. The role of the mGluR1 pathway and mGluR1 mediated glutamate release in the development of synchrony will also be examined in vivo. The strength of this project is the combination of multi-electrode electrophysiology, molecular dissection of the mGluR1 pathway in astrocytes, and computational analyses.
Delaware State University is an equal opportunity, Title IX Employer and does not discriminate against persons on the basis of race, religion, national origin, sexual orientation, gender, marital status, age or disability.
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